# Chromatography

We are searching data for your request:

Forums and discussions:
Manuals and reference books:
Data from registers:
Wait the end of the search in all databases.
Upon completion, a link will appear to access the found materials.

## Chromatography parameters for characterizing two separate analytes

### Resolution R.

$R.=2tr2−tr1w1+w2=1,18tr2−tr1w0,51+w0,52tR. - (gross) retention timew - base peak widthw0,5 - Peak width at half height$
• Measure of the system's ability to separate two analytes under the chosen chromatographic conditions.
• A resolution larger is sufficient $1,5$ (at $1,5$ only overlap 0.3% of the peak areas).

### Selectivity factor (separation factor) α

$α=K2K1=k2'k1'K - partition coefficientk' - capacity coefficient$
• Measure of the separability of two analytes.
• The greater the difference in the distribution coefficients, the easier it is to separate.
• Since the substance eluted later is always at the top in the quotient, the selectivity factor is always greater than one.
• Values ​​greater than 1 to 10 are optimal.